Table 2.

Relative Conversion of Various Glucosinolates to Hydrolysis Products by Arabidopsis ESP Expressed in E. coli ^a

Glucosinolate	Hydrolysis Product	Relative Conversion
2-Hydroxy-3-butenyl	3-Hydroxy-4,5-epithiobutyl nitrile	100
Allyl (2-propenyl)	3,4-Epithiobutyl nitrile	66
4-Methylsulfinylbutyl	5-Methylsulfinylpentyl nitrile	35
3-Hydroxypropyl	4-Hydroxybutyl nitrile	30

^a Assays were performed in 100 mM Mes buffer, pH 6.0, in the presence of myrosinase. The rate of 2-OH-3-butenyl glucosinolate conversion (0.34 μmol per 30-min assay per resuspended pellet from 180 mL of bacterial culture) was set to 100.