Figure 1.

CRIB Motif–Dependent Specific Interaction of RIC1 with the GTP-Bound Active Rop.

In vitro pulldown assays were performed using MBP-RIC1 and GST–CA-rop1 or GST–DN-rop1, and MBP-RICs pulled down by GST-rop1 were detected by anti-MBP antibody as described in the text.

(A) In vitro interaction of RIC1 with GTP-bound CA-rop1 and GDP-bound DN-rop1 mutant proteins.

(B) In vitro interaction of the GTP-bound CA-rop1 mutant with RIC1 and RIC mutant proteins. H37D and H37D/H40D mutations are within the CRIB motif.