Figure 6.

Differential in Vitro Interaction of Rop1 with Different RICs.

GTP-bound GST–CA-rop1 fusion and indicated MBP-RIC fusion proteins were expressed in E. coli, purified by affinity chromatography, and used for pulldown assays as described in Figure 1. Only seven of the nine RICs were used for this assay because we were unable to express the other two RICs (RIC3 and RIC10) as MBP fusion proteins in E. coli. At top is shown MBP-RIC fusion proteins that bind to GST–CA-rop1; at bottom is shown the loading control for MBP-RIC fusion proteins used for the pulldown assay. Numbers between the rows indicate relative signal intensities for CA-rop1–bound MBP-RICs standardized with the loading control (see text).