TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant genotype or characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu7697) galU rpsL endAl nupG | Invitrogen |
| S. salivarius ATCC 25975 | Wild type, Lac+ Glu+ Gal+ | 12 |
| S. thermophilus SMQ-301 | Wild type, Lac+ Glu− Gal− | 32 |
| Plasmids | ||
| pUC18 | Cloning vector, AprlacZ′ (lacZα) | 37 |
| pCR-Blunt | Cloning vector | Invitrogen |
| PCR 2.1 TOPO | Cloning vector, Apr KarlacZα | Invitrogen |
| pGAL1 | Contains the last 1,040 nucleotides of galT and the first 526 nucleotides of galE from S. salivarius cloned into pCR-Blunt | This work |
| pGAL3 | Contains the last 650 nucleotides of galK and the first 1,202 nucleotides of galT from S. salivarius cloned into pCR-Blunt | This work |
| pGAL29 | Contains the first 882 nucleotides of galK and the first 853 nucleotides of galR from S. salivarius cloned into PCR 2.1 TOPO | This work |
| pGAL73 | Contains the last 1,422 nucleotides of dexB, galR, and the first 729 nucleotides of galK from S. salivarius cloned into pUC18 | This work |
| pGAL123 | Contains the last 104 nucleotides of galT, galE, and the first 928 nucleotides of galM from S. salivarius cloned into pUC18 | This work |