FIG. 1.

Subcellular localization of the β-subunit of DNA polymerase III and the SeqA protein in exponentially growing cells. Cells of strain YK1100 (11) growing in M9 medium supplemented with 0.5% glucose and 50 μg of l-tryptophan per ml at 37°C were fixed and analyzed by indirect immunofluorescence microscopy (4) with rabbit anti β-subunit of DNA polymerase III polyclonal antibodies and rabbit anti-SeqA polyclonal antibodies. (A) Subcellular localization of the β-subunit of DNA polymerase III (left). Pictures on the right show 4′,6′-diamidino-2-phenylindole (DAPI)-stained cells. (B) Subcellular localization of the SeqA protein (left). Pictures on the right show DAPI-stained cells.