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. 2002 Feb;184(3):867–870. doi: 10.1128/JB.184.3.867-870.2002

FIG. 3.

FIG. 3.

Comparison of subcellular localization between the β-subunit of DNA polymerase III and the SeqA protein in cells synchronized for initiation of chromosome replication. To synchronize the initiation of chromosome replication, cells of the temperature-sensitive dnaC mutant strain (strain PC2) were exponentially grown in L medium supplemented with thymine (50 μg/ml) at 30°C and then transferred sequentially to 42, 30, and 42°C as shown in arrows. Cells were removed, fixed, and analyzed by indirect immunofluorescence microscopy. (A) Subcellular localization of the β-subunit of DNA polymerase III (left). Pictures on the right show 4′,6′-diamidino-2-phenylindole (DAPI)-stained cells. (B) Subcellular localization of the SeqA protein (left). Pictures on the right show DAPI-stained cells.