FIG. 1.

Extracellular protein profile of S. fredii USDA257 and USDA257Mu78 (nolX mutant). Extracellular proteins were prepared from cells grown in the absence (lanes 1 and 3) or presence (lanes 2 and 4) of 1 μM genistein. The proteins were resolved on an SDS-15% polyacrylamide gel and stained with Coomassie brilliant blue (A) or electrophoretically transferred to nitrocellulose for immunological analysis with NolX-specific antibodies (B). Lanes 1 and 2, extracellular proteins from USDA257; lanes 3 and 4, extracellular proteins from USDA257Mu78 (nolX mutant); lanes 5, molecular size markers. The numbers between the panels indicate the sizes of the proteins in kilodaltons. The arrow in panel B points to NolX.