FIG. 5.

Immunogold localization of NolX in mature nodules. Thin sections of 20-day-old soybean (A) and cowpea (B) nodules incubated with NolX-specific antibodies and protein A-gold revealed no specific labeling either on the bacteria or in the space enclosed by the peribacteroid membrane. A few randomly occurring protein A-gold particles can be seen (arrowheads). Note the presence of densely packed poly-β-hydroxybutyrate granules within the bacteroids. CW, cell wall; B, bacteroid; PBM, peribacteroid membrane; PHB, poly-β-hydroxybutyrate granule. Bars, 0.5 μm.