TABLE 5.
Growth on CN-Cbl of the Δpfs::Km and ΔyadS::Km mutants
| Strain | Growtha on:
|
||||||
|---|---|---|---|---|---|---|---|
| Met | Cn-Cbl at (nM):
|
||||||
| 0 | 0.5 | 5 | 50 | 500 | 5,000 | ||
| RK4353 (wild type) | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
| NC13 (RK4353 Δpfs) | ± | − | − | − | − | − | − |
| NC15 (RK4353 ΔyadS) | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
| RK4379 (metE) | ++ | − | + | ++ | ++ | ++ | ++ |
| NC16 (RK4379 Δpfs) | ± | − | − | − | − | − | − |
| NC18 (RK4379 ΔyadS) | ++ | − | + | ++ | ++ | ++ | ++ |
| RK4936 (btuB) | ++ | − | − | − | − | ± | + |
| NC19 (RK4936 Δpfs) | ± | − | − | − | − | − | − |
| NC21 (RK4936 ΔyadS) | ++ | − | − | − | − | ± | + |
| RK5015 (tonB) | + | − | − | − | − | − | + |
| NC22 (RK5015 Δpfs) | ± | − | − | − | − | − | − |
| NC24 (RK5015 ΔyadS) | + | − | − | − | − | − | + |
| RK6049 (btuC) | ++ | − | − | + | + | + | + |
| NC25 (RK6049 Δpfs) | + | − | − | − | − | − | − |
| NC27 (RK6049 ΔyadS) | ++ | − | − | + | + | + | + |
a
Growth is indicated relative to that of the parental strain on minimal medium with ammonium as a nitrogen source (++).