FIG. 2.

Physical map of the 7.2-kb orf358-containing SacI fragment of R. gelatinosus. Genes and ORFs are represented by arrows and named above in italics. Solid lines above the map represent inserts used to construct pSO67 and pCm358. Converging arrows represent the oligonucleotides used in the PCR screen. The restriction enzyme site used to insert the Km cartridge into pSO67 to construct SIX9Km is indicated below.