FIG. 4.
Spectroscopic analysis of Bchl a precursors purified by HPLC from WT or SIX9Km cultures grown under high oxygenation. (A) UV/Vis absorption spectra; (B) fluorescence emission spectra. The absorbance λmax were used as excitation wavelengths for fluorescence measurements. Solid lines, WT fraction 2; dotted and dashed lines, SIX9Km fractions 1 and 3, respectively. The solvent is acetonitrile-methanol-dichloromethane (75:15/:5, vol/vol/vol). Fraction numbers refer to Table 2. Zero levels were shifted for better viewing.