TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| E. coli XL1-Blue | supE44 hsdR17 recA1 endA1 gyrA46 thi-1 relA1 lac F′ [proAB+lacIqlacZΔM15 Tn10 (Tcr)] | Stratagene |
| R. gelatinosus | ||
| S1 | Wild type | 32 |
| SIX9Km | Interposon strain (orf227::Km at StuI) | This work |
| SIX9Km+pCm358 | SIX9Km bearing the replicative plasmid pCm358 | This work |
| SIX9Km+pTc | SIX9Km bearing the replicative plasmid pBBR1MCS3 | This work |
| Plasmids | ||
| pUC4K | Plasmid bearing the Km cartridge (Apr Kmr) | Pharmacia |
| pBluescript KS(+) | Cloning vector | Stratagene |
| pBBR1MCS1 | Cloning vector (bom+ Cmr) | 19 |
| pBBR1MCS3 (pTc) | Cloning vector (bom+ Tcr) | 19 |
| pBBR1MCS4 | Cloning vector (bom+ Apr) for R. gelatinosus S1 genomic DNA library | 19 |
| pCm358 | A 1.69 kb HindIII-XmnI fragment from pKS1-21 comprising orf154 and orf358 is inserted at HindIII/SmaI in pBBR1MCS1 | This work |
| pB1-21 | pBBR1MCS4-based vector, isolated from our genomic DNA library, contains a 10-kb insert including crtB | This work |
| pSO67 | pBluescript KS(+) where a 1.34-kb HindIII-BglII fragment containing orf154 and most of orf358 is inserted at HindIII/BamHI | This work |
| pSO67Km | Suicide vector derived from pSO67, where the Km cartridge is inserted at StuI to disrupt orf358 | This work |
a
Km, kanamycin resistance cartridge; Apr, ampicillin resistant; Cmr, chloramphenicol resistant; Kmr, kanamycin resistant; Tcr, tetracycline resistant.