TABLE 2.

HPLC analysis of pigments extracted from R. gelatinosus WT or SIX9Km cultures^a

Fraction no.	RTb (min)	λmax in HPLC solventc	Compound identificationd	Detectione in:
				WT	SIX9Km
1	2.7	416, 552, 590	MgPMe	−	+
2	3.0	438, 576, 626	Protochlorophyllide a	+	−
3	4.2	402, 504, 538, 574, 630	Protoporphyrin IX	−	+
4	5.5	530 (560)	Diketospirilloxanthin	+	+
5	6	482 (508)	OH-spheroidenone	+	+
6	10.8	770	Bchl a	+	−
7	11.2	418, 442, 472	OH-neurosporene	+	−
8	12.3	482 (508)	Spheroidenone	+	+
9	15.0	430, 458, 488	Spheroidene	−	±
10	22.0	418, 442, 472	Neurosporene	±	±

^aCultures were grown under high oxygenation in the dark. The HPLC solvent was acetonitrile-methanol-dichloromethane (75:15:15, vol/vol/vol).

^bRT, retention time.

^cDetermined in the UV/Vis range. Values in parentheses correspond to shoulders on the spectra.

^dBchl a precursors were identified according to the methods in references 4, 6, and 22.

^e+, detected; ±, detected in trace amounts; −, not detected.