TABLE 1.
Bacterial strains and cosmids or plasmids used in this study
| Strain, cosmid, or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| SM10 | Mobilizing strain: transfer genes of RP4 integrated into the chromosome; Kmr Sms | 11 |
| TOP10 | High-efficiency transformation strain with blue/white screening; Aps Kms | Invitrogen |
| HB101 | Serum-sensitive strain | 7 |
| B. mallei | ||
| NCTC 120 | USAMRIIDa | |
| NCTC 10248 | Human isolate | USAMRIID |
| NCTC 10229 | USAMRIID | |
| NCTC 10260 | Human isolate | USAMRIID |
| NCTC 10247 | USAMRIID | |
| ATCC 23344 | Human isolate; Pmr Gms | USAMRIID |
| NCTC 3708 | Mule isolate | USAMRIID |
| NCTC 3709 | Horse isolate | USAMRIID |
| ATCC 10399 | USAMRIID | |
| ATCC 15310 | USAMRIID | |
| PB100 | ATCC 23344::pGSV3008; Pmr Gmr | This study |
| B. pseudomallei 1026b | Clinical isolate; Gmr Kmr Smr Pmr Tps | 4 |
| Cosmids | ||
| pScosBC1 | Broad-host-range cosmid cloning vector based on pSuperCos1; Apr Tpr | 12 |
| p1C3 | pScosBC1 from ATCC 23344 library with a 23-kb fragment containing the O-PS biosynthetic gene cluster | This study |
| p2B5 | pScosBC1 from ATCC 23344 library with a 27-kb fragment containing the O-PS biosynthetic gene cluster | This study |
| Plasmids | ||
| pUC19 | Cloning vector with blue/white selection; Apr | 14 |
| pGSV3008 | pGSV containing a 379-bp EcoRI fragment from pDD3008, contains internal fragment from the wcbB gene; Gmr | 6 |
a
U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Md.