FIG. 2.

In vivo and in vitro oligomerization of Pal. In vivo experiments were carried out with the lpp-ompA JC8963 strain. Western blotting using anti-Pal antibodies or Coomassie blue staining are shown. (A) In vivo and in vitro cross-linking with FA cross-linked samples were treated at 37°C (FA) and 96°C (FA96). (B) In vivo cross-linking of lpp-ompA cells with FA, EGS, or DST. Control cells were treated at 37°C (C) and 96°C (C96). Pal multimers (P2 and P3) and size markers (in kilodaltons) are indicated.