TABLE 1.
Bacterial strains used in this studya
| Strain (original designation) | Relevant genotypeb | Source, reference, or construction |
|---|---|---|
| SJW1103 | Wild type | 39 |
| SE5221 | lgt(Ts) | 12 |
| SE5312 | lnt(Ts) | 13 |
| FDY3 (SE5221 lpp::Tn10) | lgt(Ts) lpp::Tn10 | 12 |
| FDY110 | lgt(Ts) lpp::Tn10 | P22(FDY3 × SE5221)c; this work |
| FDY7 (SE5312 zbf::Tn10) | lnt(Ts) zbf::Tn10 | 13 |
| FDY8 (SE5312 lpp::Tn10) | lnt(Ts) lpp::Tn10 | 13 |
| FDY40 | lnt(Ts) lpp | Tets of FDY8; this workd |
| FDY43 | lnt+zbf::Tn10 | P22(FDY7 × SJW1103); this work |
| FDY46 | lnt+lpp::Tn10 | P22(FDY43 × FDY40)c; this work |
| FDY50 | lnt+lpp | Tets of FDY46; this work |
| FDY113 | lgt(Ts) lpp | Tets of FDY110; this work |
| FDY115 | lgt(Ts) lnt(Ts) lpp zbf::Tn10 | P22(FDY7 × FDY113); this work |
| MH111 | fliC::Tn10 | M. Homma |
| FDY82 | lnt(Ts) lpp fliC::Tn10 | P22(MH111 × FDY40); this work |
| SJW1684 | fliF | S. Yamaguchi |
| KK1113 | fliF-lacZ | 25 |
| FDY105 | lnt(Ts) lpp fliF-lacZ | P22(KK1113 × FDY40); this work |
| FDY106 | lnt+lpp fliF-lacZ | P22(KK1113 × FDY50); this work |
| KK1107 | flhD-lacZ | 25 |
| FDY107 | lnt(Ts) lpp flhD-lacZ | P22(KK1107 × FDY40); this work |
| FDY108 | lnt+lpp flhD-lacZ | P22(KK1107 × FDY50); this work |
| SJW1469 | flgH | S. Yamaguchi |
| FDY126 | lgt(Ts) lpr-1 | Revertant of SE5221; this work |
| FDY127 | lgt(Ts) lpr-2 | Revertant of SE5221; this work |
| FDY131 | lpp::Tn10 of FDY126 | This work |
| FDY147 | lpp::Tn10 of FDY127 | This work |
| FDY9 | pLnt (lnt+)e | Tetr of SE5312 (13) |
| FDY4 | pSK004dp (lgt+)f | Ampr of SE5221 (12) |
| FDY135g | pFDY135 (lnt+)e | Ampr of DH5α; this work |
| FDY140g | pFDY140(lgt+)f | Ampr of DH5α; this work |
| FDY137 | pFDY135/lnt(Ts) | Ampr of SE5312; this work |
| FDY138 | pFDY135/lnt(Ts) lpp | Ampr of FDY8; this work |
| FDY144 | pFDY140/lgt(Ts) | Ampr of SE5221; this work |
| FDY145 | pFDY140/lgt(Ts) lpr-1 | Ampr of FDY126; this work |
| FDY146 | pFDY140/lgt(Ts) lpr-2 | Ampr of FDY127; this work |
| TOP1Og | Δ(mrr-hsdRMS-mcrBC) | Invitrogen |
| DH5αg | BBL |
All strains used in this study are derived from S. enterica serovar Typhimurium LT2, except where indicated otherwise.
Genotype is chromosomal, except when preceded by a plasmid name, in which case it is the plasmid-encoded genotype.
P22 transductions were done as described in reference 10 using P22HT105 int 1 phage.
Tets strains were selected on Bochner plates as described in reference 5.
pLnt is based on the medium-copy-number plasmid pACYC184 (Tetr). A 2.3-kb EcoRI fragment carrying the wild-type lnt allele from pLnt was subcloned into the low-copy-number plasmid pWSK29 (Ampr) (38) to give pFDY135.
pSK004dp is based on the high-copy-number plasmid pBluescript SK(+) (12). A 1.4-kb SalI-Xba I fragment containing the wild-type lgt allele was subcloned into the low-copy-number plasmid pWSK29 (Ampr) (38) to give pFDY140.
E. coli strains.