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. 2002 Feb;184(3):812–820. doi: 10.1128/JB.184.3.812-820.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 2. — PCR analysis of glnKY51F amtB3::Tet transformants. (A) Large- and small-colony Tet^r Amp^s transformants of wild-type strain UW136. (Top) amplified PCR products; (bottom) products after EcoRI digestion. EcoRI cleaves glnKY51F in half, while glnK is EcoRI resistant. Lanes: 1 to 5, small-colony transformants; 6 to 9, large-colony isolates; 10, glnKY51F PCR product amplified from plasmid pPR118; 11, glnK amplified from plasmid pPR101. 12, Amp^r Tet^r isolate resulting from integration of plasmid pPR118 carrying both alleles in tandem. (B) Transformation of glnD1::Ω suppressor strain MV72. Lanes: 1 to 5, Tet^r Amp^s isolates; 6, glnKY51F amplified from plasmid pPR118; 7, wild-type glnK from pPR101.