TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and/or characteristics | Reference or source |
|---|---|---|
| Strains | ||
| A. vinelandii | ||
| UW136 | Rifr derived from strain UW (ATCC 13705) | 7 |
| UW136Ha | nifH1-lacZ/KSS | This work; 46 |
| MV71 | gln-71 glnD::Ω | 11 |
| MV72 | gln-71 (spontaneous glnD1::Ω suppressor) | 11 |
| MV378 | nifL1::KIXX nifH1-lacZ/KSS | 6 |
| MV577 | gln-71 glnKY51F amtB3::Tetr | This work |
| MV577H | gln-71 glnKY51F amtB3::TetrnifH1-lacZ/KSS | This work |
| MV578 | gln-71 amtB3::Tetr | This work |
| MV578H | gln-71 amtB3::TetrnifH1-lacZ/KSS | This work |
| MV579 | gln-71 glnKY51F amtB3::TetrnifL1::KIXX | This work |
| MV579H | gln-71 glnKY51F amtB3::TetrnifL1::KIXX nifH1-lacZ/KSS | This work |
| MV580 | gln-71 amtB3::TetrnifL1::KIXX | This work |
| MV580H | gln-71 amtB3::TetrnifL1::KIXX nifH1-lacZ/KSS | |
| MV581 | gln-71 nifL1::KIXX | This work |
| MV581H | gln-71 nifL1::KIXX nifH1-lacZ/KSS | |
| E. coli | ||
| DH5α | supE44 hsdR17 recA1 thi-1 ΔlacU169 (φ80lacZΔM15) endA1 gyrA96 relA1 | 18 |
| MC4100 | [Δ(lac)U169 araD139 rpsL thi] | 10 |
| CK1005 | MC4100 with (ΔglnB)::Camr | |
| CK1007 | MC4100 with (ΔglnB)::CamrglnK::Spcr | This work |
| CK1008 | MC4100 with (ΔglnB)::CamrglnK::SpcrglnD99::Tn10 | This work |
| NCM1971 | glnK::Spcr | 20 |
| NCM1686 | glnD99::Tn10 | 19 |
| NCM1736 | (ΔglnB)::Camr | 19 |
| S. cerevisiae | ||
| SFY526 | UAS-GAL1-lacZ reporter (His− Trp− Leu−) | Clontech |
| HF7c | UASG 17-mer(X3)CYC1-lacZ UAS-GAL1-HIS3 | Clontech |
| Plasmids | ||
| pAB29 | pTZ19 derivative carrying carrying nifL1::KIXX | 6 |
| pBR322 | Tetr vectors source of Tetr gene | 9 |
| pGAD424b | GAL4 activation domain fusion two-hybrid vector | Clontech |
| pGBT9b | GAL4 binding domain fusion two-hybrid vector | Clontech |
| pJAW2 | Suicide plasmid carrying nifH1-lacZ/KSS | 46 |
| pPR101 | pTZ19 carrying A. vinelandii glnK amtB | 31 |
| pPR102 | pTZ19 carrying A. vinelandii glnKY51F amtB | This work |
| pPR113 | Plasmid encoding GlnKHis6 in pTrcHisB | This work |
| pPR115 | Plasmid encoding GlnKY51FHis6 in pTrcHisB | This work |
| pPR118 | pPR102 with a 1.4-kb EcoRI/AvaI Tetr gene cloned into amtB | This work |
| pTrcHis B | His tag cloning vector | Invitrogen |
| pTD1 | Activation domain control fusion for two-hybrid system | Clontech |
| pVA3 | Binding domain control fusion for two-hybrid system | Clontech |
a
Strain UW136H is a remake of strain MV101 (46).
b
For descriptions of two-hybrid fusion constructs, see Materials and Methods.