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. 2002 Feb;184(3):864–866. doi: 10.1128/JB.184.3.864-866.2002

FIG. 1.

FIG. 1.

Effect of zinc depletion on growth of E. coli W3110, GR352 (ΔznuABC::cam), and GR354 (ΔzupT::cam ΔznuABC). Growth yields with different EDTA concentrations are shown. (A) Overnight cultures grown in LB medium were diluted 1:400 into fresh mineral salts medium (12) with the indicated concentrations of EDTA. Cell growth was monitored by measuring the OD600 after 6 h of incubation at 37°C with shaking and converted to dry weight. Results are shown for E. coli W3110 (⧫), E. coli GR316 (ΔzupT::cam) (•), E. coli GR352 (ΔznuABC::cam) (▪), and E. coli GR354 (ΔzupT::cam ΔznuABC) (▴). Experiments were performed in triplicate, and the average was calculated. (B) Cells were treated as for panel A except that equimolar amounts of EDTA and metals were added (both at 250 μM). Results are shown for E. coli W3110 (light grey bars), E. coli GR352 (ΔznuABC::cam) (dark grey bars), and E. coli GR354 (ΔzupT::cam ΔznuABC) (black bars).