FIG. 2.

Growth of E. coli strains expressing zupT in the presence of metals or EDTA. Overnight cultures grown in LB medium were diluted 1:400 into fresh LB medium with the indicated concentrations of zinc (A), EDTA (B), copper (C), or cadmium (D), and expression of zupT was induced with 200 ng of AHT/ml. Growth was monitored after 6 h by measuring the OD₆₀₀ and converted to dry weight (mg/ml). (A, B, D) ▪, E. coli GG48 (ΔzntA::kan ΔzitB::cam)(pZUPT); ▴, E. coli GG48 (ΔzntA::kan ΔzitB::cam) vector control. (C) ▪, E. coli W3110(pZUPT); ▴, E. coli W3110 vector control. Results of representative experiments are shown.