FIG. 4.

rM51R-M virus induces expression of costimulatory molecules on mDC. mDC were infected with rwt or rM51R-M virus at multiplicities of 0.01, 0.1, and 1 PFU/cell or were treated with LPS or loxoribine. At 24 h postinfection or posttreatment, the cell surface expression of CD40, CD80, and CD86 was measured by flow cytometry. (A) Representative histograms depicting expression of costimulatory molecules by rwt and rM51R-M viruses (1 PFU/cell). The geometric mean fluorescence of each sample was determined and used to quantitate the increase in CD40 (B), CD80 (C), and CD86 (D) expression over that in untreated cells (NT). Data are the means ± standard errors of three or four independent experiments.