TABLE 1.
Virus | Cells | Antibody dilution | Amt of infectious virus (log10 PFU) following neutralization witha:
|
||
---|---|---|---|---|---|
Anti-HPIV3 | Anti-EV | Anti-HPIV3 + anti-EV | |||
HPIV3 | LLC-MK2 | 1:10 | <1.2 | 3.9 | <1.2 |
1:40 | <1.2 | NDb | <1.2 | ||
No antibody | 4.7 | 4.6 | 4.6 | ||
Vero | 1:10 | <1.2 | 3.9 | <1.2 | |
1:40 | <1.2 | ND | <1.2 | ||
No antibody | 4.8 | 4.8 | 4.7 | ||
HPIV3/EboGP | LLC-MK2 | 1:10 | 3.2 | <1.2 | 1.2 |
1:40 | 3.7 | 2.1 | 2.6 | ||
No antibody | 4.7 | 4.7 | 4.7 | ||
Vero | 1:10 | 3.6 | 1.7 | 1.9 | |
1:40 | 3.9 | 2.3 | 2.7 | ||
No antibody | 4.9 | 5.1 | 5.2 |
The indicated viruses (105.2 PFU) were incubated with the indicated dilution of anti-HPIV3 antibodies, anti-EV antibodies, or their mixture in the presence of complement. After the incubation, the residual infectivity was determined on day 7 by plaque titration in the indicated cell lines.
ND, not determined.