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. 2006 Mar;80(5):2170–2182. doi: 10.1128/JVI.80.5.2170-2182.2006

FIG. 5.

FIG. 5.

The DENV2 cHP directs start codon selection via a position-dependent, sequence-independent mechanism. (A) Schematic diagrams of constructs used to test the position and sequence dependence of the DENV2 cHP in regulating start site selection. (B) Anti-FLAG immunoblot of C-FLAG-transfected Hep3B cells at 4 h posttransfection. The efficiency of codon usage is expressed as a ratio of the higher-molecular-weight C-FLAG isoform (1st AUG) to the lower-molecular-weight isoform (2nd AUG). The error bars indicate SDs; the data are derived from three experiments. (C) Anti-FLAG immunoblot of transfected C6/36 cells at 24 h posttransfection as in panel B. The graph is as in panel B; the data represent the averages of three experiments. (D) Anti-FLAG immunoblot of transfected Hep3B cells at 4 h posttransfection as in panel B. The mean ratio and SD are calculated from four experiments. (E) Anti-FLAG immunoblot of transfected C6/36 cells at 24 h posttransfection as in panel B. The table is as in panel D; the data represent the averages of four experiments. The ratio of 32 reflects the maximum difference detectable by immunoblotting under the conditions described. *, P < 0.01 relative to wt.