FIG. 7.

The cHP is required for efficient DENV2 replication. In vitro-transcribed IC RNAs were transfected into Hep3B and C6/36 cell monolayers, and viral replication was assessed after 72 h by plaque assay. The titers were normalized to transfection efficiency as determined by qRT-PCR at 2 h posttransfection. (A) Schematics of IC variants utilized to study the role of the cHP and of the nucleotides that make up the DENV2 initiation context. (B) Viral titers are expressed as PFU per ml from IC-transfected Hep3B cells. One log unit reflects the limit of detection of a standard plaque assay. The error bars indicate SD; the data are derived from at least four experiments. (C) Viral titers from IC-transfected C6/36 cells. The graph is as in panel B; the data are derived from at least four experiments. (D) Viral titers from IC-transfected Hep3B cells. The graph is as in panel B; the data are derived from at least four experiments. (E) Viral titers from IC-transfected C6/36 cells. The graph is as in panel B; the data are derived from at least three experiments. †, not detectable.