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. 2006 Mar;80(5):2106–2117. doi: 10.1128/JVI.80.5.2106-2117.2006

FIG. 4.

FIG. 4.

Removal of detergent from purified PrP-res does not improve infection efficiency. SN56 cell infections were conducted and analyzed as described in the legend to Fig. 3 with the exception that the purified PrP-res was prewashed with PBS to remove the detergent. SN56 cells were treated with the indicated amounts of Chandler PrP-res as contained in scrapie (Sc+) microsomes (lanes 1 to 4) or purified from brains of infected animals (lanes 7 to 10). Some infections with purified PrP-res were supplemented with microsomes from normal (Sc−) animals (lanes 11 to 14) in quantities normalized for total protein to the corresponding scrapie microsome samples. The cells were treated with normal microsomes normalized for protein content to 20 ng (lane 5) or 4 ng (lane 6) of scrapie microsomes as a control. At the indicated passages (A and B), the cells were assayed (100 μg protein/lane) by immunoblotting for PrP-res. Lane numbers in panel B apply to panel A also. (C) Quantitation of PrP-res in panel B. The results are expressed as percent PrP-res signal for each purified PrP-res sample (lanes 7 and 8 and 11 to 13) relative to an infection initiated with the same amount of PrP-res as contained in scrapie brain microsomes. The PrP-res level for each scrapie microsome infection was set at 100%. The error bars indicate the range (n = 2).