TABLE 2.

Numbers of molecules per cell at 24 hpi and leader types on sgmRNAs

Genomea	No. of viral genomesb	No. of DI RNA genomesb	No. of sgmRNA moleculesb	Molar ratio of sgmRNA to genome	Type of leader on sgmRNA
Viral	20		70 (sgmRNA 5)	3.5	65-nt wt leader (from 12.7 IS2 site)c
Wt12.7 DI RNA		97	16	0.2	65-nt wt leader (from 12.7 IS2 site)c
M24 DI RNA		155	0	0	No sgmRNA by Northern analysis and no 5′ RACE product
WtX DI RNA		44	4	0.1	33-nt minileaderc,e
M11 DI RNA		130	14	0.1	56-nt minileaderc
M15 DI RNA		137	32	0.2	65-nt wt leaderc
M17 DI RNA		38	7	0.2	65-nt wt leaderc
M34 DI RNA		149	18	0.1	65-nt wt leaderc
M43 DI RNA		260	0	0	No sgmRNA by Northern analysis and no RT-PCR product
Viral	20		2,000 (sgmRNA 7)	100	65-nt wt leader (from N IS site)c
WtN DI RNA		17	13	0.8	65-nt wt leader (from N IS site)c
WtN-M1 DI RNA		30	4	0.1	34-nt minileaderd
					65-nt wt leader (from sgmRNA 7)c
WtN-M2 DI RNA		75	2	0.03	60-nt minileaderd
					65-nt wt leader (from sgmRNA 7)c

^aGenomes were either viral or mutant DI RNA generated from plasmid DNA.

^bDetermined from Northern analyses and the specific activity of the probe at 24 hpi with virus alone or with first-passage virus (VP1) following transfection with DI RNA transcripts. For the virus genome and sgmRNAs 5 and 7, the Northern probe detecting an N gene-specific sequence was used, and for DI RNA and DI RNA-derived sgmRNAs, the Northern probe detecting an HSV gD reporter-specific sequence was used.

^cDetermined by sequencing cloned RT-PCR products using leader16(−) as one of the primers.

^dDetermined by sequencing cloned RT-PCR products using leader11(−) as one of the primers.

^eDetermined by sequencing cloned 5′ RACE products.