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. 2006 Mar;80(5):2445–2452. doi: 10.1128/JVI.80.5.2445-2452.2006

FIG. 1.

FIG. 1.

Overexpression of ATM enhances HIV-1 replication in a kinase-independent manner. (a) 293T cells (2 × 105 cells) were cotransfected with an HIV-1 molecular clone (1 μg) and wild-type (wt) or kinase-defective (kd) ATM-expressing plasmid or the empty vector pcDNA3-FLAG (2 μg). Three days after transfection, p24 levels in the culture supernatant were measured by p24 ELISA. (b) Overexpression of ATM enhances VSV G-pseudotyped HIV-1 production. 293T cells (2 × 105 cells) were cotransfected with HIV-1 packaging construct pCMV R8.91 (2 μg), HIV-GFP (WPTS-GFP) (1 μg), VSV G-envelope-expressing plasmid (pMDG) (1 μg), and wt or kd ATM-expressing plasmids or the empty vector pcDNA3-FLAG (2 μg). Three days after transfection, p24 levels in the culture supernatant were measured by ELISA.