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Overexpression of ATM enhances Rev function. 293T cells (2 × 104 cells) were cotransfected with the Rev-dependent luciferase-based reporter gene pDM628 (100 ng), the Rev-expressing plasmid pcRev (100 ng), and/or wild-type (wt) or kinase-defective (kd) ATM-expressing plasmids (200 ng). Twenty-four hours after transfection, luciferase activity in the cellular lysates was measured. Results are from three independent experiments.
