FIG. 4.

Increased accumulation of viral RNA in Ssa overexpression strains. (A) Northern blot analysis of total RNA extracts for DI-72 RNA accumulation at 12 (lanes 1, 5, and 9), 18 (lanes 2, 6, and 10), 24 (lanes 3, 7, and 11), and 36 (lanes 4, 8, and 12) h after induction using the 3′ end of DI-72 as a probe. See further details in the legend to Fig. 3. (B) Western analysis of p33 and p92 replicase proteins in total protein samples using anti-His antibody. Further details are as for Fig. 3. Note that the overexpressed Ssa1p carried a six-His tag, which was detected by the anti-His antibody, whereas the wt Ssa1p was not detected (lanes 1 to 4).