FIG. 7.

Binding of soluble NCR to vaccinia virus-infected cells. (A) HFFF2 fibroblasts were either mock infected or infected with vaccinia virus WR (multiplicity of infection = 5) for 16 h. The cells were recovered with PBS-2 mM EDTA and stained with the indicated Ig fusion protein or control Ig-Fc immobilized on fluorescent microspheres. The data are representative of more than five experiments done with two batches of the various fusion proteins. The mean fluorescence intensity of staining with each NCR-Ig fusion protein is indicated. (B) Fibroblasts were either mock infected or infected, recovered as for panel A, and then stained with the NKp30 ligand-specific MAb, 138.