FIG. 2.

Detection of HCV genomes and replicative-intermediate RNAs in the lobular region of a human liver biopsy specimen by strand-specific ISH. Biopsies fixed and frozen in OCT buffer were stained by ISH using the same HCV riboprobes as in Fig. 1, as described in Materials and Methods. Panels A and B show detection of HCV genomes with an antisense (AS) riboprobe and of HCV replicative-intermediate RNA with a sense (S) riboprobes, respectively. RNA signals are red, and cell nuclei are green. Magnification, ×40. Panels C and D illustrate negative results when staining a liver biopsy specimen obtained from an HCV-negative individual with HCV S and AS riboprobes. Magnification, ×10.