FIG. 6.

Identification of HCV core and NS3 antigens within liver tissues and HCV replicon cell lines by immunocytochemistry. Panels A and B: Huh7-replicon cells stained with anticore and anti-NS3 antibodies, respectively, followed by detection with Vector red (VR; red signal). Panels C and D: Huh7 cells without replicons stained with same antibodies, followed by Vector red detection (no red signals). Panels E and F demonstrate anticore and anti-NS3 staining of a liver biopsy specimen obtained from an HCV/HIV-coinfected individual, whereas panels G and H show negative staining using the same antibodies on a different specimen. Magnification, ×40.