FIG. 2.

Analysis of UL6, UL17, and UL25 in wt HSV-1 strain 17, wt strain KOS, lacZ-UL6⁻, KUL25NS, and UL17-stop capsids by Western blotting. Serial twofold dilutions of wt and mutant capsids that had been equalized on the basis of their VP23 content were prepared. The polypeptides were resolved by SDS-PAGE and blotted onto a nitrocellulose membrane. The blots were probed sequentially with antibodies UL6 MAb 175, UL17 MAb 203, UL25 MAb 166, and VP23 antibody Rab186 as indicated on the left side. In this experiment the UL25NS B capsid preparation was banded twice on a sucrose gradient to ensure minimal contamination with UL25NS empty capsids, lacking the scaffolding proteins.