FIG. 2.

In vitro ɛ RNA binding activity of HBV RT mutants as detected by the gel mobility shift assay. Purified HBV RT proteins, all as GST fusion proteins, were incubated with ³²P-labeled HBV ɛ RNA in the presence of the Hsp90 chaperone factors as detailed in Materials and Methods. The binding reaction mixtures were resolved on a native polyacrylamide gel, which was then dried and subjected to autoradiography. The labeled free ɛ RNA and the RT-ɛ complex are indicated.