FIG. 2.
Cell surface expression of gHHR2-up, gHHR2-down1, and gHHR2-down2 mutants and wt gH. (A to D) Paraformaldehyde-fixed COS cells transfected with wt gH or mutant gH plasmids and a gL-encoding plasmid and reacted with MAb 53S. Positive reactivity denotes gH-gL heterodimer formation and no major defect in trafficking to the plasma membrane. (E) Quantification of cell surface expression (Expr.) of the gH mutants in COS cells cotransfected with a gL plasmid by CELISA. Each assay was performed in triplicate. Bars represent mean percentages relative to wt gH ± standard deviations.