FIG.2.

Control of host cell gene expression following EBOV and MARV infection. Panel A, Venn diagram showing the segregation of infection-regulated genes that showed a ≥2-fold (n = 4; P < 0.01) change in expression at 24 h p.i. Hierarchical clustering matrix of the expression of genes in set I that were preferentially regulated by ZEBOV at 24 h, in set II that were commonly regulated by ZEBOV and MARV, in set III that were common to all viral infections, and in set IV that were restricted to REBOV. Panel B, Venn diagram showing the segregation of infection-regulated genes that showed a ≥2-fold (n = 4; P < 0.01) change in expression at 48 h p.i. and the associated hierarchical clustering diagrams of genes in sets I to IV. Panel C, matrix showing the expression of selected inflammation- and apoptosis-related genes in ZEBOV-, REBOV-, and MARV-infected cells at 24 and 48 h p.i. In the left panel, genes shown in red were up-regulated and genes shown in green were down-regulated relative to uninfected Huh7 cells, while black indicates no change in gene expression. In the right panel, genes whose regulation showed a P value of >0.01 are shown in gray. Panel D, bar graphs comparing the expression of selected mRNAs as measured by quantitative real-time PCR and cDNA expression microarray analysis. The results are presented as the log ratio of mRNA abundance in infected relative to mock-infected cells.