Table 2.

Arabidopsis Growth Stages for the Soil-Based Phenotypic Analysis Platform

		Col-0 Data
Stage	Description	Daysa	sd	CVb
Principal growth stage 1	Leaf development
1.02	2 rosette leaves >1 mm in length	12.5	1.3	10.7
1.03	3 rosette leaves >1 mm in length	15.9	1.5	9.5
1.04	4 rosette leaves >1 mm in length	16.5	1.6	9.8
1.05	5 rosette leaves >1 mm in length	17.7	1.8	10.2
1.06	6 rosette leaves >1 mm in length	18.4	1.8	9.8
1.07	7 rosette leaves >1 mm in length	19.4	2.2	11.1
1.08	8 rosette leaves >1 mm in length	20.0	2.2	11.2
1.09	9 rosette leaves >1 mm in length	21.1	2.3	10.8
1.10	10 rosette leaves >1 mm in length	21.6	2.3	10.9
1.11	11 rosette leaves >1 mm in length	22.2	2.5	11.2
1.12	12 rosette leaves >1 mm in length	23.3	2.6	11.3
1.13	13 rosette leaves >1 mm in length	24.8	3.2	12.8
1.14	14 rosette leaves >1 mm in length	25.5	2.6	10.2
Principal growth stage 3	Rosette growth
3.20	Rosette is 20% of final size	18.9	3.0	16.0
3.50	Rosette is 50% of final size	24.0	4.1	17.0
3.70	Rosette is 70% of final size	27.4	4.1	15.0
3.90	Rosette growth complete	29.3	3.5	12.0
Principal growth stage 5	Inflorescence emergence
5.10	First flower buds visible	26.0	3.5	13.3
Principal growth stage 6	Flower production
6.00	First flower open	31.8	3.6	13.3
6.10	10% of flowers to be produced have opened	35.9	4.9	13.6
6.30	30% of flowers to be produced have opened	40.1	4.9	12.3
6.50	50% of flowers to be produced have opened	43.5	4.9	11.2
6.90	Flowering complete	49.4	5.8	11.7
Principal growth stage 8	Silique ripening
8.00	First silique shattered	48.0	4.5	9.3
Principal growth stage 9	Senescence
9.70	Senescence complete; ready for seed harvest	NDc	ND	ND

^a Average day from date of sowing, including a 3-day stratification at 4°C to synchronize germination.

^b CV, coefficient of variation, calculated as (sd/days) × 100.

^c ND, not determined (see text for details).