Table 3.
Measurements Performed during the Plate-Based Phenotypic Analysis
First-Phase Measurements
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Measurement/Query | Growth Stage Defined | |||||
Has radicle emergence been reached or passed? | Stage 0.5 | |||||
Are the hypocotyl and cotyledons visible? | Stage 0.7 | |||||
Have the cotyledons opened fully? | Stage 1.0 | |||||
Number of rosette leaves >1 mm | Principal growth stage 1 | |||||
Length of primary root ≥6 cm
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Stage R6
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Second-Phase Measurements
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Col-0 Data
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Measurement | Unit | Growth Stage | Average | sd | CVa | |
Number of rosette leaves | Count | 1.0b | 3.3 | 0.5 | 13.7 | |
Length of primary root | mm | 1.0b | 45.2 | 4.1 | 9.0 | |
Number of secondary roots | Count | R6c | 10.5 | 1.4 | 13.2 | |
Rosette, total exposed leaf area | mm2 | R6c | 22.2 | 2.6 | 11.9 | |
Rosette, perimeter | mm | R6c | 42.1 | 5.2 | 12.2 | |
Rosette, sd of radius | None | R6c | 39.2 | 3.1 | 7.9 | |
Rosette, major axis | mm | R6c | 7.9 | 0.7 | 9.1 | |
Rosette, minor axis | mm | R6c | 5.9 | 0.6 | 9.5 | |
Rosette, eccentricity | None | R6c | 0.63 | 0.05 | 7.2 |
a CV, coefficient of variation, calculated as (sd/days) × 100.
b Data collection initiated at stage 1.0 and continued until the end of the experiment.
c R6 or 14 days after sowing, whichever comes first.