Table 4.
Measurements Performed during the Soil-Based Phenotypic Analysis
| First-Phase Measurements
| |||||
|---|---|---|---|---|---|
| Measurement/Query | Growth Stage Defined | ||||
| Rosette radius | Principal growth stage 3 | ||||
| Are flower buds visible? | Stage 5.10 | ||||
| Is first flower open? | Stage 6.00 | ||||
| Length of stem | Stage 6.50a | ||||
| Number of open flowers | Principal growth stage 6 | ||||
| Number of senescent flowers | Principal growth stage 6 | ||||
| Number of filled siliques | Principal growth stages 6 and 7 | ||||
| Number of shattered siliques | Principal growth stage 8 | ||||
| Is flower production complete?
|
|
|
|
Stage 6.90
|
|
| Second-Phase Measurements
| |||||
| Col-0 Data
|
|||||
| Measurement | Unit | Growth Stage | Average | sd | CVb |
| Number of cotyledons | Count | 1.04 | 2.0 | 0.1 | 5.0 |
| Rosette, total exposed leaf area | mm2 | 1.10 | 580.0 | 202.2 | 34.9 |
| Rosette, perimeter | mm | 1.10 | 418.0 | 119.1 | 28.5 |
| Rosette, sd of radius | None | 1.10 | 45.7 | 3.2 | 7.0 |
| Rosette, major axis | mm | 1.10 | 40.4 | 8.0 | 19.8 |
| Rosette, minor axis | mm | 1.10 | 34.6 | 7.5 | 21.7 |
| Rosette, eccentricity | None | 1.10 | 0.5 | 0.1 | 20.0 |
| Rosette, total exposed leaf area | mm2 | 6.00 | 3225.0 | 1088.3 | 33.7 |
| Rosette, perimeter | mm | 6.00 | 808.1 | 181.3 | 22.4 |
| Rosette, sd of radius | None | 6.00 | 36.7 | 3.5 | 9.5 |
| Rosette, major axis | mm | 6.00 | 82.3 | 15.3 | 18.6 |
| Rosette, minor axis | mm | 6.00 | 73.1 | 13.4 | 18.3 |
| Rosette, eccentricity | None | 6.00 | 0.4 | 0.1 | 25.0 |
| Rosette, dry weight | mg | 6.00 | 117.4 | 45.9 | 39.1 |
| Number of stem branches on main bolt | Count | 6.50 | 3.4 | 0.6 | 17.6 |
| Number of side bolts >1 cm | Count | 6.50 | 4.2 | 1.2 | 28.6 |
| Length of peduncle of second flower on main bolt | mm | 6.50 | 11.5 | 1.6 | 13.9 |
| Distance across face of open flower | mm | 6.50 | 3.9 | 0.3 | 7.7 |
| Sepal length | mm | 6.50 | 2.2 | 0.2 | 9.1 |
| Pollen grain, area | μm2 | 6.50 | 589.0 | 132.0 | 22.4 |
| Pollen grain, perimeter | μm | 6.50 | 114.5 | 13.0 | 11.2 |
| Pollen grain, sd of radius | None | 6.50 | 9.1 | 2.5 | 27.5 |
| Pollen grain, major axis | μm | 6.50 | 30.6 | 3.3 | 10.8 |
| Pollen grain, minor axis | μm | 6.50 | 24.3 | 3.0 | 12.3 |
| Pollen grain, eccentricity | None | 6.50 | 0.6 | 0.1 | 16.7 |
| Silique, area | mm2 | 6.50 | 10.6 | 1.9 | 17.9 |
| Silique, perimeter | mm | 6.50 | 40.9 | 6.1 | 14.9 |
| Silique, sd of radius | None | 6.50 | 55.8 | 0.5 | 0.9 |
| Silique, major axis | mm | 6.50 | 17.2 | 1.7 | 9.9 |
| Silique, minor axis | mm | 6.50 | 1.2 | 0.2 | 16.7 |
| Silique, eccentricity | None | 6.50 | 1.0 | 0.0 | 0.0 |
| Total number of seeds per silique valve | Count | 6.50 | 29.9 | 2.8 | 9.4 |
| Number of abnormal seeds per silique valve | Count | 6.50 | 0.2 | 0.4 | 200 |
| Dry weight of stem | mg | 6.50 | 188.8 | 39.3 | 20.8 |
| Dry weight of rosette | mg | 6.50 | 163.7 | 52.0 | 31.8 |
| Total number of siliques | Count | 6.90 | 160.4 | 60.7 | 37.8 |
| Seed, area | mm2 | 9.70 | 0.14 | 0.01 | 7.1 |
| Seed, perimeter | mm | 9.70 | 1.95 | 0.04 | 2.1 |
| Seed, sd of radius | None | 9.70 | 16.92 | 0.94 | 5.6 |
| Seed, major axis | mm | 9.70 | 0.53 | 0.03 | 5.7 |
| Seed, minor axis | mm | 9.70 | 0.33 | 0.02 | 6.1 |
| Seed , eccentricity | None | 9.70 | 0.78 | 0.02 | 2.6 |
| Seed yield per plant (desiccated) | mg | 9.70 | 127.9 | 52.7 | 41.2 |
a Used to define the working definition of stage 6.50 as described in the text.
b CV, coefficient of variation, calculated as (sd/days) × 100.