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. 2006 Mar;80(6):2784–2796. doi: 10.1128/JVI.80.6.2784-2796.2006

FIG. 2.

FIG. 2.

Comparison of CNS infection with different viruses in 6-day-old NIH Swiss mice. Six-day-old mice (10 per group) were inoculated i.c. or s.c. with 20 μl of PBS containing 5 × 106 PFU of TC83 or chimeric SIN/VEE viruses. (A) Animal mortality following i.c. inoculation with chimeric SIN/VEE viruses or TC83. To evaluate the virulence of chimeric SIN/VEE viruses in comparison to the vaccine strain, TC83, mice were inoculated with SIN/TRD, SIN/ZPC, SIN83, SAAR/TRD, or TC83 and monitored twice daily for 2 months, and deaths were recorded. (B) Viral replication in the brain. Mice infected with parent or chimeric viruses via i.c. route (shown in panel A) were sacrificed (three per group) on days 1 to 5 postinfection. Brains were harvested, and homogenized tissues were used in a plaque assay performed in BHK-21 cells to quantify viral titers (PFU per gram of brain tissue). (C) Animal mortality following s.c. inoculation with TC83 or chimeric SIN/VEE viruses. To evaluate the safety of chimeric SIN/VEE viruses in comparison to that of TC83, mice were inoculated via s.c. route with 5 × 106 PFU of SIN/TRD, SIN/ZPC, SIN83, SAAR/TRD, or TC83 and monitored twice daily for 2 months, and deaths were recorded.