TABLE 1.
Titers of neutralizing antibody in vaccinated mice
| Virus strain | Titer of antibody (mean ± SD) aftera:
|
|||
|---|---|---|---|---|
| Single immunizationb
|
Booster immunizationc
|
|||
| 4 wk p.i. | 8 wk p.i. | 4 wk p.i. | 8 wk p.i. | |
| SIN83 | 55 ± 73 | 73 ± 76 | 100 ± 40 | 160 ± 120 |
| SIN/TRD | 37 ± 23 | 57 ± 27 | 50 ± 55 | 73 ± 47 |
| SAAR/TRD | 126 ± 53 | 167 ± 89 | 160 ± 113 | 152 ± 107 |
| SIN/ZPC | 187 ± 109 | 253 ± 205 | 253 ± 106 | 487 ± 254 |
a
The reciprocal titer of the serum capable of neutralizing 80% of PFU of TC83 virus in a plaque reduction neutralization test.
b
Six-week-old, female NIH Swiss mice (12 per virus strain) were inoculated on day 0 s.c. into the medial thigh with the indicated chimeric SIN/VEE virus at a dose of 5 × 105 PFU in a total volume of 100 μl of PBS.
c
Six-week-old, female NIH Swiss mice (six per virus strain) received an additional booster on day 28 s.c. into the medial thigh with the indicated chimeric SIN/VEE virus at a dose of 5 × 105 PFU in a total volume of 100 μl of PBS.