TABLE 2.

Challenge studies to determine protection against clinical encephalitis and death in mice vaccinated with chimeric SIN/VEE viruses

Vaccine groupa	Clinical outcome after inoculation challenge (no. of mice)b
	Encephalitis			Death
	s.c.	i.c.	i.n.	s.c.	i.c.	i.n.
SIN83	0	2	1	0	1	0
SIN/TRD	0	0	0	0	0	0
SIN/ZPC	0	0	0	0	0	0
SAAR/TRD	0	0	0	0	0	0
Mockc	5	5	5	5	5	5

^aSix-week-old, female NIH Swiss mice (15 per virus strain) were inoculated on day 0 s.c. into the medial thigh with the indicated chimeric SIN/VEE virus at a dose of 5 × 10⁵ PFU in a total volume of 100 μl of PBS or PBS alone.

^bEncephalitis (paralysis and/or ataxia) or death following s.c. challenge (five per group) with ZPC738 at a dose of 10⁶ PFU per animal in 100 μl and i.c. (five per group) or i.n. (five per group) challenge with ZPC738 at a dose of 2 × 10⁵ PFU per animal in 20 μl of PBS at 8 weeks following vaccination with the indicated chimeric SIN/VEE virus.

^cPBS alone.