FIG. 2.

A representative HMA of the 5′ half-genome of ON2, ON4, and IV3. PCR products from different weeks postinfection were digested with AseI and prepared for HMA as described in Materials and Methods. The AseI sites and resultant fragment sizes are shown schematically at the top of the figure in relation to the genomic structure. Each fragment is given a letter designation to indicate its position on the gel. Heterogeneity at the AseI site that flanked fragment C in pol resulted in different fragment sizes and is indicated as C to C2. Heteroduplex formation occurred in fragments B and D in o.n. inoculated cats, and AseI fragment C was the most common. In contrast, sequences from IV3 had variants C and C2, which were identified at each time point. Heteroduplex formation in all fragments was more noticeable in all IV3 PCRs except at the week 37 time point. The numbers at the top of the gel indicate the week of the sample, and the lowercase designation represents an individual PCR product.