FIG. 7.
Effect of suppressing endogenous RNase L activity on the growth of WNV in MEFs. Cells were infected with WNV at an MOI of 5 (A), an MOI of 1 (B), or an MOI of 0.05 (C), and virus growth kinetics were compared. Samples of culture fluid were harvested at the indicated times, and titers were determined by plaque assay on BHK cells. ST/neo, susceptible He MEFs stably transfected with the empty expression vector pcDNAI/Neo. RT/neo, resistant RV MEFs stably transfected with the empty expression vector pcDNAI/Neo. RT/ZB1-83, resistant RV MEFs stably expressing dominant negative mutant RNase L from pDNAI-ZB1. The virus titers at each time point are the averages of results of duplicate titrations from two experiments. Error bars indicate standard deviation and are shown where appropriate. The asterisk indicates titers below the detection limit of 102 PFU/ml.