Figure 6.

Effect of a Deleterious Mutation in the ACT7 Gene on Hormone-Induced Callus Formation.

Mutant samples are shown in the left panels and wild-type samples are shown in the right panels.

(A) to (G) Young cotyledons of almost similar size before ([A] and [B]) and after 7 days ([C] and [D]) and 21 days ([E] to [G]) of hormone treatment. (F) and (G) show enlarged cotyledon-derived calli from (E).

(H) and (I) Leaf explants after 21 days of incubation on CIM. Similar sized leaves were used for callus regeneration.

(J) to (L) Root explants after 21 days of incubation on CIM.

Bars in (A) to (D) = 1 mm; bars in (E) and (J) = 10 mm; bars in (F), (G), (H), (I), (K), and (L) = 5 mm.