Figure 8.

Complementation of act7-1 Plants with the ACT7 Gene Sequence Reverses the Slow Regeneration of Callus to the Normal Wild-Type (WT) Level.

Forty milligrams of young leaves was incubated on CIM, and after 21 days the fresh weight of the leaf explants was measured. Comp1 and Comp2 represent two independent complemented lines. Note that the callus regenerated from act7-1 mutant leaves had approximately 30% less weight than the wild-type leaf callus. Leaves from the complemented plants and act2-1 mutants produced callus almost equal to the wild-type leaves. The mean values of three different experiments with standard errors are shown.