FIG. 5.

Inhibition of HIV virus production by PBS zinc fingers in primary cells. (A) Flow cytometry analysis of zinc finger expression in PBMCs. PBMCs were transduced with lentiviral vectors that express KRAB-PBS zinc finger proteins. Cells were fixed and stained with an antibody that recognizes the C-terminal HA tag of the ZFs and analyzed by flow cytometry for zinc finger expression. The right panel shows flow cytometry analyses of KRAB-PBS2-transduced cells stained for CD4 and HA antibodies. (B) PBMCs were transduced with the indicated KRAB-PBS lentiviral vector and then challenged with infection by the HIV-1 strain NL4-3 at an MOI of 0.1. p24 levels were monitored at 3, 7, 12, and 18 days after infection.