FIG. 8.

Mutation of the primer-binding site in response to zinc finger repression. (A) PBMCs were transduced with KRAB-PBS2 and infected with HIV-1. Virus was collected from the supernatant and used to reinfect a new population of transduced cells. After several rounds, output virus was cloned, and the primer-binding site regions from 20 clones were sequenced. The sites of mutation are indicated by underlining. (B) Infection of KRAB-PBS-transduced PBMCs by wild-type and Mut-3 HIV. (C) Gel shift of PBS2 with wild-type and Mut-3 hairpin oligonucleotides.