TABLE 2.

Properties of the original and recombinant virus strains

Virus	Virus titer			Leak yield			Total RNA synthesis at 39°C (%)	Defecta		RNA phenotypea
	28°C	39°C	Ratio (39°C/28°C)	Titer at 28°C	Titer at 39°C	Ratio (39°C/28°C)		26S RNA	P1234 processing
wt	1.8 × 109	1.3 × 109	0.72	7 × 107	1.3 × 108	1.9	100	−	−	+
SFowt	2.6 × 109	1.4 × 109	0.54	2.5 × 108	1.1 × 109	4.4	100	−	−	+
ts1	6.8 × 108	<2.5 × 101	<3.7 × 10−8	1.4 × 107	4 × 102	2.8 × 10−5	88	+	−	+
SFons1	1.5 × 109	4.5 × 108	0.3	2.8 × 108	1.8 × 108	0.64	96	+	−	+
ts4	4 × 108	5 × 104	1.3 × 10−4	6.8 × 107	5.7 × 104	8.4 × 10−4	1	+	+	−
SFots4	2 × 109	3.8 × 105	1.9 × 10−4	3.0 × 108	2 × 105	6.7 × 10−4	<0.5	+	+	−
ts6	2 × 108	9.7 × 104	4.9 × 10−4	2.9 × 109	7 × 105	2.4 × 10−4	2.5	+	+	−
SFots6	4.2 × 108	1.6 × 106	3.8 × 10−3	1.8 × 108	3.5 × 107	0.19	35	+	+	±
ts9	3.3 × 108	2.2 × 104	6.7 × 10−5	1.4 × 108	4.7 × 104	3.4 × 10−4	3	+	−	−
SFots9	2.5 × 109	1.3 × 105	5.2 × 10−5	6.5 × 108	1.5 × 105	2.3 × 10−4	0.5	+	−	−
ts10	1.7 × 108	<2.5 × 101	<1.5 × 10−7	1.0 × 108	1.6 × 104	1.6 × 10−4	6	−	−	±
SFons10	1.5 × 109	5.8 × 108	0.39	4. × 108	6.3 × 108	1.6	10	−	−	±
ts11	5 × 108	4.5 × 105	9 × 10−4	5.5 × 107	7 × 103	1.3 × 10−4	2.5	±	+	−
SFots11	2 × 109	7 × 106	3.5 × 10−3	2.6 × 108	2.5 × 105	9.6 × 10−4	1	±	+	−
ts13	1.2 × 108	<2.5 × 101	<2 × 10−7	8.8 × 106	1.1 × 104	1.3 × 10−3	3	+	ND	−
SFons13ab	2.2 × 109	1.4 × 106	6.4 × 10−4	1.8 × 109	5.5 × 104	3 × 10−5	1.5	+	ND	−
SFons13a	8 × 108	6.3 × 107	7.9 × 10−2	1.2 × 108	7.7 × 108	1.6	17	NDb	ND	±
SFons13b	6 × 108	2.2 × 108	0.37	3 × 108	4.8 × 108	6.4	51	ND	ND	+
ts14	1.4 × 108	2.1 × 103	1.5 × 10−5	3.4 × 107	9 × 105	2.6 × 10−2	<0.5	−	−	−
SFots14	1.9 × 109	1.2 × 106	6.3 × 10−4	3.6 × 108	1.6 × 105	4.4 × 10−4	0.5	−	−	−

^aThe criteria for different classes are defined in the text.

^bND, not determined.