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Stability of ETIF in RK13 cells infected with complemented vL11ΔETIF using an MOI of 20. ETIF was detected with anti-ETIF MAb L3ab at the indicated times p.i. in infected-cell lysates before and after intensive washing steps. Full-length ETIF is indicated by a solid arrow. The membrane was stripped and incubated with a control anti-β-actin MAb in a second reaction. The sizes of the PageRuler Prestained Protein Ladder (Fermentas) are given in thousands.
